[Python] Why does my cv2.imread() giving me None? (python)

I am new to this image detection thing and I've been following a youtube video and trying to make a program that detects an image so I can automate some fishing in a game. But I keep getting this error where everytime I made a correct path to the image file I keep getting None output. Could somebody help me with this please?

from numpy import asarray
from mss import mss
import cv2
import time
import pyautogui
import imageio.v3 as iio

import os
import shutil

def vision():
cords = (400,400,1100,400)
mark = cv2.imread("D:\\Python\\New folder\\apple.png")

print(cv2.imshow("OpenCV Image",mark))

while True:
vision()


My screenshot of the output:



expected the image to be read but when I recall it, it wont work.

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