[Python] When I edit a .env file, and try to use the new variable the old one gets loaded

This is the content of .env file

YOUTUBE_API_KEY=SyCnieNj
# old-api-key
# YOUTUBE_API_KEY=AIzayCno


When i try to use YOUTUBE_API_KEY the old api is loaded This is the part of the python code:

import os
import json
import time
import googleapiclient.discovery
import googleapiclient.errors
from dotenv import load_dotenv

# Load API keys
load_dotenv()

# YouTube API setup
YOUTUBE_API_KEY = os.getenv("YOUTUBE_API_KEY")


Even after removing all the comment line from .env file os.getenv("YOUTUBE_API_KEY") is still loading old api key

Can anyone explain the issue here?

When I changed the api key in .env and reloaded the script the script is still using the old api key

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