[Python] PYGAME Circle changes colour on clicking but changes back on mouse up

Now I know that this is due to how I have blocked my code. Clicking on any of the circles changes them to black, on release they go back to yellow. I want them to stay black. I can see why they go back to yellow - makes perfect sense, but how can I stop it doing it?

I am quite happy for the answer to be "no hope from here..."! I'm very much a beginner I cannot be patronised, lol!

import pygame
import random

pygame.init()

#Display Window
SCREEN_HEIGHT = 800
SCREEN_WIDTH = 1400

#Colours
BLUE = (0, 0, 255)
LIGHTBLUE = (72, 243, 235)
BLUEBLACK = (20, 21, 77)
IODINE = (243, 200, 99)
REDYELLOW = (221, 78, 20)
BLACK = (0, 0, 0)

#Location of top left of well plate, diameter of wells, default colour of well, horizontal and vertical spacing
# between wells.
x = 500
y = 400
size = 40
wellcolour = IODINE
colour = IODINE
spacing = size*2.3
yspacing = size*2.3
start = True
clicked = 0


screen = pygame.display.set_mode((SCREEN_WIDTH, SCREEN_HEIGHT))
pygame.display.set_caption('Amylase')
#icon = pygame.image.load("plant.png")
#pygame.display.set_icon(icon)

class Experiment():
def __init__ (self, colour, x, y, size, number):


self.rect = pygame.Rect(x-size, y-size, size*2, size*2)
pygame.draw.rect(screen, LIGHTBLUE, self.rect)
pygame.draw.circle(screen, colour, (x, y), size )
pygame.draw.circle(screen, BLACK, (x, y), size, 4)
if self.rect.collidepoint(mouse_pos) and event.type == pygame.MOUSEBUTTONDOWN:
colour = BLACK
pygame.draw.circle(screen, colour, (x, y), size )





#!!!!!!!GAME LOOP!!!!!
run =True



while run:



mouse_pos = pygame.mouse.get_pos()
#print(mouse_pos)


for event in pygame.event.get():


if event.type == pygame.QUIT:
run = False



screen.fill(LIGHTBLUE)



#Row one
Experiment(wellcolour, x, y, size, 1)
Experiment(wellcolour, x+spacing, y, size, 2)
Experiment(wellcolour, x+2*spacing, y, size, 3)
Experiment(wellcolour, x+3*spacing, y, size, 4)
Experiment(wellcolour, x+4*spacing, y, size, 5)

#Row two
Experiment(wellcolour, x, y+yspacing, size, 6)
Experiment(wellcolour, x+spacing, y+yspacing, size, 7)
Experiment(wellcolour, x+2*spacing, y+yspacing, size, 8)
Experiment(wellcolour, x+3*spacing, y+yspacing, size, 9)
Experiment(wellcolour, x+4*spacing, y+yspacing, size, 10)

#Row three
Experiment(wellcolour, x, y+2*yspacing, size, 11)
Experiment(wellcolour, x+spacing, y+2*yspacing, size, 12)
Experiment(wellcolour, x+2*spacing, y+2*yspacing, size, 13)
Experiment(wellcolour, x+3*spacing, y+2*yspacing, size, 14)
Experiment(wellcolour, x+4*spacing, y+2*yspacing, size, 15)

#Row four
Experiment(wellcolour, x, y+3*yspacing, size, 16)
Experiment(wellcolour, x+spacing, y+3*yspacing, size, 17)
Experiment(wellcolour, x+2*spacing, y+3*yspacing, size, 18)
Experiment(wellcolour, x+3*spacing, y+3*yspacing, size, 19)
Experiment(wellcolour, x+4*spacing, y+3*yspacing, size, 20)


pygame.display.flip()

pygame.quit()

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