I have this code #Plot # Define a custom palette for the strains custom_palette = { 'H9CK': 'green', 'H9RT': 'blue', 'H9TK': 'red', 'H9WD': 'orange' } # Define a custom hatching pattern for each strain hatch_patterns = { 'H9CK': '//', # Diagonal lines 'H9RT': '\\\\', # Opposite diagonal lines 'H9TK': '--', # Horizontal dashes 'H9WD': 'xx' # Cross hatching } # Plot plt.figure(figsize=(10, 6)) boxplot = sns.boxplot( data=subset_df, x='dpi', y='ΔΔCt', hue='strain', palette=custom_palette, showfliers=False ) # Add hatching patterns to the boxes for i, patch in enumerate(boxplot.patches): # Determine the strain for the current box strain = subset_df['strain'].unique()[i % len(hatch_patterns)] patch.set_hatch(hatch_patterns[strain]) # Add labels and title plt.title('Boxplot of ΔΔCt by dpi and strain') plt.xlabel('DPI (Days post-infection)') plt.ylabel('ΔΔCt') # Move the legend to the upper right corner plt.legend(title='Strain', loc='upper right', bbox_to_anchor=(1.05, 1), borderaxespad=0.) # Adjust layout plt.tight_layout() # Show the plot plt.show() which is giving me this kind of plots enter image description here Why and how do I fix that the pattern is different on day number 1? Thank you all! I want it to have the same patterns Continue reading...