I am trying to call a Python file "hello.py" from within the python interpreter with subprocess. But I am unable to resolve this error. [Python 3.4.1]. import subprocess subprocess.call(['hello.py', 'htmlfilename.htm']) Traceback (most recent call last): File "<pyshell#42>", line 1, in <module> subprocess.call(['hello.py', 'htmlfilename.htm']) File "C:\Python34\lib\subprocess.py", line 537, in call with Popen(*popenargs, **kwargs) as p: File "C:\Python34\lib\subprocess.py", line 858, in __init__ restore_signals, start_new_session) File "C:\Python34\lib\subprocess.py", line 1111, in _execute_child startupinfo) OSError: [WinError 193] %1 is not a valid Win32 application Also is there any alternate way to "call a python script with arguments" other than using subprocess? Continue reading...