This is an example image of what i am trying to analyze: The yellow line is the line tool in imageJ. I would take a "plot profile" which would give me the value of the pixels over the line. I have an image that contains various stripes. They are very close together at the top and progressively get further apart. I want to create a program, in Python, that will identify these stripes and take a line through each one and retain the pixel value in a 1D array. I would like to see how the line matches the image, so it would ideally include an overplot. I am currently using the line tool in ImageJ and manually drawing each line-out. I would like to automate this process. Continue reading...