My Intention was: - Extract all frames from video - Apply background removal to each frame - Merge all frames together as a video with transparency Problem: - The resulting video doesn't have the desired transparency where the background used to be My code for the Merging-Part import cv2 import os image_folder = r"C:\Users\youre\Documents\machine_learning\bg-rm\output" video_name = r'C:\Users\youre\Documents\machine_learning\bg-rm\test.avi' images = # list of all frames frame = cv2.imread(os.path.join(image_folder, images[0])) height, width, layers = frame.shape video = cv2.VideoWriter(video_name, cv2.VideoWriter_fourcc(*"MJPG"), 30, (width, height)) for image in images: video.write(cv2.imread(os.path.join(image_folder, image))) cv2.destroyAllWindows() video.release() My question: - What do I have to do in order to have my transparency over the background in the resulting video, because my code just gives me a black background. More specific: How do I merge the png-frames together with an alpha channel? Continue reading...