I have tried different tutorials in order to create a single multilayer TIFF from individual files, but with none of them I managed to open the file correctly with Adobe PhotoShop. Below is an example of what I tried. I can open the combined TIFF with GIMP, where I can "see" individual pages that can be imported as layers, but PhotoShop just imports a single layer. I saw a thread on a similar subject, but I think there was no conclusion about how to solve this issue. path = "img-tif" in_paths = [(path + "/000{:d}.tif".format(i)) for i in range(5)] images = [tifffile.imread(p) for p in in_paths] out_path = "img-test/multi.tif" with tifffile.TiffWriter(out_path) as tiff: for i, img in enumerate(images): tiff.write(img, photometric='rgb', contiguous=False, #planarconfig='separate', compression='none', subfiletype=1 if i > 0 else 0, predictor=False, metadata={'LayerName': f'Layer_{i+1}', 'DocumentName': 'Multilayer TIFF', 'Software': 'Python tifffile'}) Do you have any suggestion? Thanks in advance. Continue reading...